ABSTRACT
Background: hydatidosis is one of the zoonotic diseases which affect animals and human beings at the larval stage of Echinococcusgranulosus, thereby playing a role in exacerbating the economic and health problems in Iran
Objectives: this study was conducted to investigate the strains of Echinococcusgranulosus isolated from cattle hydatid cysts, in Khorramabad, in the Lorestan Province, in the west of Iran
Methods: twenty-six isolates of hydatid cyst of cattle from lung [24 samples] and liver [2 sample] organs were collected from Golshan Slaughterhouse, in Khorramabad. All of the samples were transferred to the laboratory for morphometric characterization and molecular study. In morphological characterization, blade length of large [LBL] and small [SBL] hooks and the ratio of blade length to total length in large [LBL/LTL] and small [SBL/STL] hooks and total length of large [LTL] and small [STL] hooks were measured. In molecular study, a partial sequence of cytochrome oxidase 1 [CO1] with 440 bp in length was amplified applying primers J3 and J4.5. Genomic DNA sequencing was performed by Sanger's method
Results: the morphological results showed that there is no significant difference between isolated from cattle hydatid cyst and sensu stricto strain [p<0.05]. The results of molecular studies support the findings of morphological characterization. All sequences showed 100% identity with sensu stricto strain
Conclusions: the results from this study showed that sensu stricto strain [G1] is a causative agent of cattle hydatid cyst in Khorramabad. On the other hand, the cattle play a role in enabling the parasite to complete its cycle. Hence in order to execute a control program for minimizing the effects of this disease, the cattle should be considered as a source of infection for dogs. The results of this study could be helpful in designing such control program in the region
ABSTRACT
Leucocytozoonosis is a disease of birds caused by obligate intracellular protozoa of the genus Leucoytozoon. We determined the prevalence of Leucocytozoon spp. using light and transmission electron microscopy in domestic birds in southwest of Iran. A total of 825 blood smears from 275 birds were examined for presence of infection. The structure morphology of "Leucocytozoon spp. was studied using light and electron microscopy. Forty-four [16.0%] of the birds were positives for Leucocytozoon. The detected parasite were found in 14 chickens [5.1%], 12 geese [4.3%], 10 ducks [3.6%], and 8 turkeys [2.9%]. The majority of the records were from the northeastern regions. Leucocytozoonosis are distributed in the Lorestan province bird population and electron microscopy can resolve the problem to distinguish between similar species of Leucocytozoon
ABSTRACT
The presence of Visceral Larva Migrans [VLM] in a patient is reported. A 57-year-old woman suffering from right upper abdominal and suprapubic pain referred into a clinic in Khorramabad, Lorestan Province, Iran. A cystoscopy was performed and biopsy was taken. The light microscopic study showed a couple of larvae as well as mononuclear inflammatory cell-infiltration. Because occurrence of VLM is potentially problem in rural areas, it is recommended that an educational program to be initiated to prevent and control VLM infection in both rural and urban people. Clinicians also should consider the clinical features of visceral larva migrans
Subject(s)
Humans , Female , Urinary Bladder/parasitology , Abdominal Pain , Cystoscopy , ToxocaraABSTRACT
The aim of this study was to assess the effects of exogenous histamine and H[1] and H[2] central receptors on electrocardiographic [ECG] parameters, heart rate and mean electrical axis in broiler chickens. For this purpose, Ross broiler chickens [750-800 g weight] received intracerebroventricularly [ICV] histamine. Moreover, to determine the receptors involved in histamine-induced alterations in ECG changes, H[1] and H[2] blockers were administered to histamine-treated chickens. All ECGs were standardized at 1 mV = 20 mm, with paper speed of 50 mm/sec. Leads I, II, III, aVR, aVL and aVF were recorded. Injection of histamine [200 and 300 micro g] decreased the duration of T wave and QRS complex and elevated the heart rate as compared to the control group [P<0.05]. Chlorpheniramine [100 micro g], an H[1] receptor antagonist, increased the duration rate of T wave and QRS complex and reduced the heart rate [P<0.05]. Cimetidine, an H[2] receptor antagonist [100 micro g] had no effect on ECG parameters. Pretreatment with chlorpheniramine [100 micro g] significantly attenuated histamine effects [200 micro g] on the duration of T wave, QRS complex and heart rate [P<0.05]. Mean electrical axis, calculated from leads II and III, did not differ between groups and it was between -93 [degree sign] and -99 [degree sign] in all chickens. In conclusion, exogenous histamine intracerebroventricularly results in sinusoidal tachycardia in broiler chickens and central H[1] receptors mediate the stimulatory effects of histamine on heart rate, but H[2] receptors had no role in this manner
ABSTRACT
This study was conducted to investigate the possible involvement of the histaminergic system in the mediation of ghrelin-induced feeding behavior in broilder chickens. In the trial 1, the effect of intracerebroventricular [ICV] injection of ghrelin on feed intake was examined in 3-h feed-deprived broiler chickens. In the trials 2 and 3, the chickens were received ICV chlorpheniramine [an H[1] receptor antagonist] was measured at 2-h post injections. Infusion of ghrelin [0.3, 0.6 and 1.2 nmol] decreased feed intake dose-dependently [P<0.05]. Pre-treatment of chlorpheniramine [100 micro g] attenuated the inhibitory effect of ghrelin results suggest that there is an interaction between ghrelin and the histaminergic system [through H[1] receptors] on control of feed intake in broiler chickens
ABSTRACT
Aegyptianellosis is a disease caused by small intraerythrocytic inclusions which is restricted to the area of Africa, Asia and extreme southern Europe. In this study the prevalence of Aegyptianella spp. was evaluated in four genera of domestic poultry in the northern, southern and central regions of Lorestan province from April to September 2008. A total of 275 native adult birds including chickens, ducks, geese, and turkeys which were bred in free range pasture condition were used for blood sampling in the rural areas of the regions. Twenty one [7.6%] out of 275 birds used in this study were infected with the organisms. The detected Aegyptianella were found in 7 chickens [33.3%], 2 ducks [9.5%], 7 geese [33.3%], and 5 turkeys [23.9%], respectively. The majority of the records were from the northeastern regions. Therefore, more than one-half of the infected birds with the Aegyptianella species were located in these regions. The structure morphology of Aegyptianella spp. was studied using light and electron microscopy. The results of the study by electron microscopy demonstrated the developmental stage as well as implemented similar and different extra/intra genus
ABSTRACT
Ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. Animals in group 1 were immunized two times by whole gut homogenate [WGH] of Haemonchus contortus emulsified in Freund's adjuvant. In group 2 [control], animals were injected by phosphate buffered saline emulsified in the same adjuvant. Animals were challenged by 10000 third-stage larvae [L3] of Haemonchus contortus on day 33 after the first immunization and then humanely killed on day 42. Animals were tested for serum antibody and eggs per gram of faeces [EPG] throughout the study and nematodes in their abomasom were counted after necropsy. The results indicated that animals immunized with WGH showed a higher level of serum antibodies. A significant difference was observed in mean optical density of sera in ELISA between the two groups [P<0.05] and a 77 and 78% reduction in EPG and nematode counts at necropsy, respectively [P<0.05]
Subject(s)
Female , Animals , Sheep , Immunization , Enzyme-Linked Immunosorbent Assay , VaccinationABSTRACT
The present study was done from winter 2003 until spring 2004. Faecal Samples[n = 400] were collected from calves and cattle in four age groups:0-6,7-12,13-24 months and 2-10 years old.Sample were stained by modified Ziel-Nelson method and oocytes of cryptosporidium were diagnosed on the basis of their sporozoiets. Infection rate was equal amomg animals during all seasons. Adult cattle had the highest infection rate in the healthy ones [23.75%]. on the other hand, the highest prevalence rate of infection in the diarrheic animals was seen in the calves. While bovine cryptosporidiosis accompanying is with diarrhea in the < 6 months of age, it has not any clinical signs in the adult ones.The present animals in Khorram-Abad[17.5%] were infected